ABSTRACT
Pheromones are specialized chemical messengers used for inter-individual communication within the same species, playing crucial roles in modulating behaviors and physiological states. The detection mechanisms of these signals at the peripheral organ and their transduction to the brain have been unclear. However, recent identification of pheromone molecules, their corresponding receptors, and advancements in neuroscientific technology have started to elucidate these processes. In mammals, the detection and interpretation of pheromone signals are primarily attributed to the vomeronasal system, which is a specialized olfactory apparatus predominantly dedicated to decoding socio-chemical cues. In this mini-review, we aim to delineate the vomeronasal signal transduction pathway initiated by specific vomeronasal receptor-ligand interactions in mice. First, we catalog the previously identified pheromone ligands and their corresponding receptor pairs, providing a foundational understanding of the specificity inherent in pheromonal communication. Subsequently, we examine the neural circuits involved in processing each pheromone signal. We focus on the anatomical pathways, the sexually dimorphic and physiological state-dependent aspects of signal transduction, and the neural coding strategies underlying behavioral responses to pheromonal cues. These insights provide further critical questions regarding the development of innate circuit formation and plasticity within these circuits.
Subject(s)
Pheromones , Signal Transduction , Vomeronasal Organ , Animals , Pheromones/physiology , Mice , Signal Transduction/physiology , Vomeronasal Organ/physiologyABSTRACT
The pheromonal information received by the vomeronasal system plays a crucial role in regulating social behaviors such as aggression in mice. Despite accumulating knowledge of the brain regions involved in aggression, the specific vomeronasal receptors and the exact neural circuits responsible for pheromone-mediated aggression remain unknown. Here, we identified one murine vomeronasal receptor, Vmn2r53, that is activated by urine from males of various strains and is responsible for evoking intermale aggression. We prepared a purified pheromonal fraction and Vmn2r53 knockout mice and applied genetic tools for neuronal activity recording, manipulation, and circuit tracing to decipher the neural mechanisms underlying Vmn2r53-mediated aggression. We found that Vmn2r53-mediated aggression is regulated by specific neuronal populations in the ventral premammillary nucleus and the ventromedial hypothalamic nucleus. Together, our results shed light on the hypothalamic regulation of male aggression mediated by a single vomeronasal receptor.
Subject(s)
Aggression , Vomeronasal Organ , Aggression/physiology , Animals , Hypothalamus , Male , Mice , Neurons/physiology , Pheromones/physiology , Ventromedial Hypothalamic Nucleus , Vomeronasal Organ/physiologyABSTRACT
The vomeronasal system plays an essential role in sensing various environmental chemical cues. Here we show that mice exposed to blood and, consequently, hemoglobin results in the activation of vomeronasal sensory neurons expressing a specific vomeronasal G protein-coupled receptor, Vmn2r88, which is mediated by the interaction site, Gly17, on hemoglobin. The hemoglobin signal reaches the medial amygdala (MeA) in both male and female mice. However, it activates the dorsal part of ventromedial hypothalamus (VMHd) only in lactating female mice. As a result, in lactating mothers, hemoglobin enhances digging and rearing behavior. Manipulation of steroidogenic factor 1 (SF1)-expressing neurons in the VMHd is sufficient to induce the hemoglobin-mediated behaviors. Our results suggest that the oxygen-carrier hemoglobin plays a role as a chemosensory signal, eliciting behavioral responses in mice in a state-dependent fashion.
Subject(s)
Amygdala/metabolism , Biomarkers/blood , Hemoglobins/metabolism , Sensory Receptor Cells/metabolism , Ventromedial Hypothalamic Nucleus/metabolism , Vomeronasal Organ/metabolism , Animals , Female , Hemoglobins/genetics , In Situ Hybridization/methods , Lactation , Male , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Motor Activity/genetics , Motor Activity/physiology , Proto-Oncogene Proteins c-fos/genetics , Proto-Oncogene Proteins c-fos/metabolism , Receptors, G-Protein-Coupled/genetics , Receptors, G-Protein-Coupled/metabolism , Steroidogenic Factor 1/genetics , Steroidogenic Factor 1/metabolism , beta-Globins/genetics , beta-Globins/metabolismABSTRACT
CASE: We describe a rare case of posterior root avulsion fracture of the medial meniscus in an 11-year-old boy. Previous reports have demonstrated delayed diagnosis, but in this case, multiplanar computed tomography (CT) combined with magnetic resonance imaging (MRI) enabled early diagnosis and treatment. Gradual ossification was observed after arthroscopic suture fixation, and meniscal extrusion did not progress. He returned to sports without any symptoms and showed no degenerative changes at 2.5 years postoperatively. CONCLUSION: This is the first case report of early diagnosis and time-course analysis of a rare avulsion fracture, emphasizing the usefulness of CT combined with MRI.
Subject(s)
Fractures, Avulsion , Tibial Meniscus Injuries , Child , Fractures, Avulsion/diagnostic imaging , Fractures, Avulsion/pathology , Fractures, Avulsion/surgery , Humans , Magnetic Resonance Imaging/methods , Male , Menisci, Tibial/diagnostic imaging , Menisci, Tibial/pathology , Menisci, Tibial/surgery , Tibial Meniscus Injuries/diagnostic imaging , Tibial Meniscus Injuries/pathology , Tibial Meniscus Injuries/surgeryABSTRACT
Exocrine gland-secreting peptide 22 (ESP22) is a 10-kDa protein secreted in tears of juvenile mice. ESP22 inhibits sexual behaviors in adults, leading to a reduction in reproduction rate. We herein identified the 24 amino acid sequence within ESP22 that was essential for exhibiting sexual rejection activity. This synthesizable peptide can be useful for controlling mouse overpopulation.
